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Immunohistochemical staining of human retina shows strong cytoplasmic positivity in photoreceptor segments
Immunohistochemical staining of human retina shows strong cytoplasmic positivity in photoreceptor segments
Immunohistochemical staining of human retina shows strong cytoplasmic positivity in photoreceptor segments

Anti-LRIT3 Antibody

HPA013454
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLRIT3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LRIT3 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID345193
  • Target name
    LRIT3
  • Target description
    leucine rich repeat, Ig-like and transmembrane domains 3
  • Target synonyms
    CSNB1F, FIGLER4, leucine-rich repeat, immunoglobulin-like domain and transmembrane domain-containing protein 3, fibronectin type III, immunoglobulin and leucine rich repeat domains 4, leucine-rich repeat, immunoglobulin-like and transmembrane domains 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ3SXY7
  • Protein Name
    Leucine-rich repeat, immunoglobulin-like domain and transmembrane domain-containing protein 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LRIT3 using anti-LRIT3 antibody (A10719-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRIT3 antigen affinity purified polyclonal antibody (Catalog # A10719-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRIT3 at approximately 75 kDa. The expected band size for LRIT3 is at 75 kDa.
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