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Immunofluorescent staining of human cell line RT4 shows localization to cytosol.
Immunofluorescent staining of human cell line RT4 shows localization to cytosol.
Immunofluorescent staining of human cell line RT4 shows localization to cytosol.

Anti-LRRC1 Antibody

HPA031604
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetLRRC1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LRRC1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55227
  • Target name
    LRRC1
  • Target description
    leucine rich repeat containing 1
  • Target synonyms
    dJ523E19.1; LANO; LANO adapter protein; LAP (leucine-rich repeats and PDZ) and no PDZ protein; LAP and no PDZ protein; leucine-rich repeat-containing protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BTT6
  • Protein Name
    Leucine-rich repeat-containing protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LRRC1 using anti-LRRC1 antibody (A13956-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC1 antigen affinity purified polyclonal antibody (Catalog # A13956-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRRC1 at approximately 59 kDa. The expected band size for LRRC1 is at 59 kDa.
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Immunohistochemistry analysis in human rectum and lymph node tissues using Anti-LRRC1 antibody. Corresponding LRRC1 RNA-seq data are presented for the same tissues.
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