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Anti-LRRC59 Antibody

Anti-LRRC59 Antibody

A12762
Antibodies.com
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-LRRC59 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to LRRC59.
  • Reactivity
    Human, Mouse, Rat
  • UNSPSC
    12352203

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Immunohistochemical staining of human cerebral cortex, colon, liver and lymph node using Anti-LRRC59 antibody HPA030829 (A) shows similar protein distribution across tissues to independent antibody HPA030827 (B).
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Figure 1. Western blot analysis of LRRC59 using anti-LRRC59 antibody (A09874-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC59 antigen affinity purified polyclonal antibody (Catalog # A09874-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRRC59 at approximately 35 kDa. The expected band size for LRRC59 is at 35 kDa.
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