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Anti-LRRC59 Antibody

Anti-LRRC59 Antibody

A12762
Antibodies.com
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-LRRC59 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to LRRC59.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LRRC59 using anti-LRRC59 antibody (A09874-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC59 antigen affinity purified polyclonal antibody (Catalog # A09874-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRRC59 at approximately 35 kDa. The expected band size for LRRC59 is at 35 kDa.
Product group Antibodies
Boster Bio
Anti-LRRC59 Antibody Picoband(r)A09874-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetLRRC59
  • SizePrice
Product group Antibodies
References
GeneTex
LRRC59 antibodyGTX122902
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetLRRC59
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-LRRC59 Antibody, Affinity PurifiedA305-075A
ApplicationsWestern Blot
TargetLRRC59
  • SizePrice
Product group Antibodies
RayBiotech
Anti-LRRC59 Antibody144-10022
ApplicationsWestern Blot
TargetLRRC59
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with LRRC59 Polyclonal Antibody, Unconjugated (bs-8361R) at 1:200 overnight at 4°C, DAB staining.
Product group Antibodies
Bioss Antibodies
LRRC59 Polyclonal AntibodyBS-8361R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetLRRC59
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
LRRC59 AntibodyLS-C668289
ApplicationsWestern Blot
TargetLRRC59
  • SizePrice
Immunohistochemical staining of human cerebral cortex, colon, liver and lymph node using Anti-LRRC59 antibody HPA030829 (A) shows similar protein distribution across tissues to independent antibody HPA030827 (B).
Product group Antibodies
Atlas Antibodies
Anti-LRRC59 AntibodyHPA030829
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetLRRC59
  • SizePrice