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Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.

Anti-LRRC75B Antibody

HPA002540
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetLRRC75B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LRRC75B
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID388886
  • Target name
    LRRC75B
  • Target description
    leucine rich repeat containing 75B
  • Target synonyms
    C22orf36; FAM211B; family with sequence similarity 211, member B; leucine-rich repeat-containing protein 75B; leucine-rich repeat-containing protein C22orf36; leucine-rich repeat-containing protein FAM211B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ2VPJ9
  • Protein Name
    Leucine-rich repeat-containing protein 75B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LRRC75B using anti-LRRC75B antibody (A18683-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC75B antigen affinity purified polyclonal antibody (Catalog # A18683-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRRC75B at approximately 35 kDa. The expected band size for LRRC75B is at 35 kDa.
Product group Antibodies
Boster Bio
Anti-LRRC75B Antibody Picoband(r)A18683-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetLRRC75B
  • SizePrice