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Immunohistochemical staining of human fallopian tube shows membranous positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows membranous positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows membranous positivity in glandular cells.

Anti-LRTOMT Antibody

HPA039949
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLRTOMT
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LRTOMT Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID220074
  • Target name
    LRTOMT
  • Target description
    leucine rich transmembrane and O-methyltransferase domain containing
  • Target synonyms
    CFAP111, DFNB63, LRRC51, LRRC51-TOMT, TOMT, transmembrane O-methyltransferase, leucine rich transmembrane and 0-methyltransferase domain containing
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96E66
  • Protein Name
    Leucine-rich repeat-containing protein 51
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of LRTOMT using anti-LRTOMT antibody (A13092-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human U20S whole cell lysates, Lane 7: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRTOMT antigen affinity purified polyclonal antibody (Catalog # A13092-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRTOMT at approximately 28KD. The expected band size for LRTOMT is at 28KD.
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