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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.

Anti-LSM11 Antibody

HPA039587
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetLSM11
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LSM11 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID134353
  • Target name
    LSM11
  • Target description
    LSM11, U7 small nuclear RNA associated
  • Target synonyms
    AGS8, U7 snRNA-associated Sm-like protein LSm11
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP83369
  • Protein Name
    U7 snRNA-associated Sm-like protein LSm11
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of LSM11 using anti-LSM11 antibody (A11132-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat skin tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LSM11 antigen affinity purified polyclonal antibody (Catalog # A11132-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LSM11 at approximately 68 kDa. The expected band size for LSM11 is at 40 kDa.
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Western Blot Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, HL-60 whole cell lysate, K562 whole cell lysate All lanes: LSM11 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa
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