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Immunofluorescent staining of human cell line ASC TERT1 shows localization to plasma membrane.
Immunofluorescent staining of human cell line ASC TERT1 shows localization to plasma membrane.
Immunofluorescent staining of human cell line ASC TERT1 shows localization to plasma membrane.

Anti-LSP1 Antibody

HPA071843
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetLSP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LSP1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4046
  • Target name
    LSP1
  • Target description
    lymphocyte specific protein 1
  • Target synonyms
    WP34, pp52, lymphocyte-specific protein 1, 47 kDa actin binding protein, 52 kDa phosphoprotein, F-actin binding and cytoskeleton associated protein, leufactin (leukocyte F-actin binding protein), leukocyte-specific protein 1, lymphocyte-specific antigen WP34
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP33241
  • Protein Name
    Lymphocyte-specific protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of LSP1 using anti-LSP1 antibody (A02992-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LSP1 antigen affinity purified polyclonal antibody (Catalog # A02992-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LSP1 at approximately 52 kDa. The expected band size for LSP1 is at 37 kDa.
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