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Immunohistochemical staining of human cerebral cortex shows moderate cytoplasm granular positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasm granular positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasm granular positivity in neurons.

Anti-LUZP1 Antibody

HPA028542
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLUZP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LUZP1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7798
  • Target name
    LUZP1
  • Target description
    leucine zipper protein 1
  • Target synonyms
    LUZP, leucine zipper protein 1, filamin mechanobinding actin cross-linking protein, fimbacin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86V48
  • Protein Name
    Leucine zipper protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LUZP1 using anti-LUZP1 antibody (A12616-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LUZP1 antigen affinity purified polyclonal antibody (Catalog # A12616-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LUZP1 at approximately 120 kDa. The expected band size for LUZP1 is at 120 kDa.
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