Anti-Ly-6C (T cell activating) [HK1.4]

Absolute Antibody

Anti-Ly-6C (T cell activating) [HK1.4]

9

A flow cytometry experiment on mouse cells from L-gD-1, an L cell line that expresses HSV type 1 gD, and L-gI-1, an L cell line that expresses HSV type 1 gI was preformed. In this experiment this antibody was used for reverse blocking (Johnson et al, 1993; pmid:8104217). Th1 clones from either wild-type or fyn-/- mice were used in an experiment where it was determined if this antibody could induce lysis (Lancki et al, 1995; pmid:7493751). Flow cytometry was preformed on cells from C57BL/6, DBAI2, NJ. BALB/c, PIJ, PL/J. AKRIJ. RIII, SM/J, SJL, and CBA mice using this antibody (Havran et al, 1988; pmid:3257767). Immunofluorescence was preformed on rat EL4J cells using this antibody (Fleming et al, 1993; pmid:8360469). EL4J and EUJ-Ly-6G cell lines were immunoprecipitated with this antibody (Fleming et al, 1993; pmid:8360469). Neonatal murine calvaria-derived osteoblasts were stained for flow cytometry using this antibody (Horowitz et al, 1994; pmid:7520861).

Functional Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation

Research Use Only

Monoclonal

HK1.4

Ly6c1

lymphocyte antigen 6 complex, locus C1

AA682074; AA959465; Ly6; Ly-6; Ly6c; Ly-6C; Ly-6C1; lymphocyte antigen 6 complex, locus C; lymphocyte antigen 6C1; Lymphocyte antigen Ly-6C

Mouse

IgG2a

Lymphocyte antigen 6C1

This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2c format, for improved compatibility with existing reagents, assays and techniques.

-20°C,2°C to 8°C

12352203