Anti-Ly6G/Ly6C [RB6-8C5]

Absolute Antibody

Anti-Ly6G/Ly6C [RB6-8C5]

9

This antibody is specific for murine Ly6G, a a 21-25 kDa glycosylphosphatidylinositol (GPI)-linked protein expressed by the myeloid-derived cells in a tightly developmentally-regulated manner in the bone marrow. Monocytes express Ly6G transiently during b

This antibody has been used in FACS analyses, immunoprecipitation, immunoblotting, and immunohistochemistry in various studies, such as to elucidate the origin and roles of macrophages in the post-myocardial infarction remodelling (Sager et al, 2016), to investigate the pathogenesis of primary biliary cirrhosis (Yang et al, 2016), to confirm the phenotypic dynamics of microglial and monocyte-derived cells in glioblastoma-bearing mice (Ricard et al, 2016), and to highlight an important ageing-related defect in the mucosal immune systems ability to sample lumenal antigens (Kobayashi et al, 2013). This antibody has also been used in vivo, for example, to deplete Treg cells when delineating the paralleled developments of inflammation and immunosuppression in tumor microenvironment (Yang et al, 2010), or to deplete neutrophils when underpinning a causal role for neutrophils in severe asthma (Wilson et al, 2009).

Flow Cytometry, Western Blot, ImmunoHistoChemistry, Other Application

Research Use Only

Monoclonal

RB6-8C5

Ly6g

lymphocyte antigen 6 complex, locus G

Gr; Gr-; Gr1; Gr-1; Ly-6; Ly-6G; ly-6G.1; lymphocyte antigen 6G

Mouse

IgG1

Lymphocyte antigen 6G

This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Mouse

-20°C,2°C to 8°C

12352203