Anti-Lysozyme [F9.13.7]

Absolute Antibody

Anti-Lysozyme [F9.13.7]

7

The antibody binds to lysozyme, in particular to residues H15, G16, Y20, R21, W63, R73, L75, N77, N93, K96, K97, S100, D101. The antibody partially overlaps the epitope recognized by antibody HyHEL10. Lysozyme degrades polysacchardies of various types of

The specificity of the original format of the antibody was confirmed by ELISA analysis (Lescar et al., 1993; PMID: 7680133). The structure of the Fab fragment in complex with Guinea-Fowl Lisozyme was determined (Lescar et al., 1995; PMID: 7629116). Homogeneous kinetics of Fab and Fv fragments were studied and the KD values were 0.57 +/- 0.05 nM for scFv and 1.6 +/- 0.2 nM for Fab. Since the clone and HyHEL-10 recognized a common epitope, equilibria and kinetics of the two clones were evaluated. Results showed that the clone had greater specificity than HyHEL-10 despite its lower affinity (Pons et al., 2002; PMID: 12237453). The ability of the antibody to modulate in vitro the presentation of hen egg-white lysozyme (HEL) to specific T cells was evaluated, results showed the antibody was able to enhance the HEL presentation to L3E10 T cells (Guermonprez et al., 1999).

ELISA, Other Application

ELISA; Crystallography

Research Use Only

Monoclonal

F9.13.7

Mouse

IgG1

Numida meleagris (Helmeted guineafowl)

The antibody was raised against hen egg-white lysozyme.

-20°C,2°C to 8°C

12352203