Anti-Lysozyme [F9.13.7]

Absolute Antibody

Anti-Lysozyme [F9.13.7]

7

The specificity of the original format of the antibody was confirmed by ELISA analysis (Lescar et al., 1993; PMID: 7680133). The structure of the Fab fragment in complex with Guinea-Fowl Lisozyme was determined (Lescar et al., 1995; PMID: 7629116). Homogeneous kinetics of Fab and Fv fragments were studied and the KD values were 0.57 +/- 0.05 nM for scFv and 1.6 +/- 0.2 nM for Fab. Since the clone and HyHEL-10 recognized a common epitope, equilibria and kinetics of the two clones were evaluated. Results showed that the clone had greater specificity than HyHEL-10 despite its lower affinity (Pons et al., 2002; PMID: 12237453). The ability of the antibody to modulate in vitro the presentation of hen egg-white lysozyme (HEL) to specific T cells was evaluated, results showed the antibody was able to enhance the HEL presentation to L3E10 T cells (Guermonprez et al., 1999).

ELISA, Other Application

ELISA; Crystallography

Research Use Only

Monoclonal

F9.13.7

Mouse

IgG1

Lysozyme C

Numida meleagris (Helmeted guineafowl)

The antibody was raised against hen egg-white lysozyme.

-20°C,2°C to 8°C

41116161