Anti-M2 [C2]

Absolute Antibody

Anti-M2 [C2]

9

The antibody is specific for M2-cytoplasmatic domain. M2 is a 97-amino acid protein of which approximately half (aa 47-97) comprises the cytoplasmic domain. M2 is highly conserved among virus strains.

The scFv-Fc version of the antibody was tested against HEK cells expressing full-length M2 protein. Fluorescent signals were visible lining the internal cell wall of HEK M2 cells after fixation and permeabilization. No signal was visible in HEK cells that do not express M2, or when the cells were not permeabilized. The ability of the antibody to recognize native M2 protein produced during IVA infection of MDCK cells with the H3N2 (A/PCh/1/73) and H1N1 (A/PR/8/34) strains of influenza was detected. Efficient and specific recognition of native M2 protein by the scFv-Fc fragment of the antibody was observed via fluorescent microscopy and flow cytometry (Velappan et al., 2020; PMID: 33206590).

Flow Cytometry, ImmunoFluorescence

Research Use Only

Monoclonal

C2

Matrix protein 2

Virus

-20°C,2°C to 8°C

12352203