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Western blot analysis of Mad2L2 expression in Hela cell lysate.
Western blot analysis of Mad2L2 expression in Hela cell lysate.
Western blot analysis of Mad2L2 expression in Hela cell lysate.

Anti-Mad2L2 Monoclonal Antibody

M02357
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
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Product group Antibodies
TargetMAD2L2
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Mad2L2 Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ADFO-13
  • Gene ID10459
  • Target name
    MAD2L2
  • Target description
    mitotic arrest deficient 2 like 2
  • Target synonyms
    FANCV; hREV7; MAD2 (mitotic arrest deficient, yeast, homolog)-like 2; MAD2 mitotic arrest deficient-like 2; MAD2B; MAD2-like protein 2; mitotic arrest deficient 2-like protein 2; mitotic arrest deficient homolog-like 2; mitotic spindle assembly checkpoint protein MAD2B; polymerase (DNA-directed), zeta 2, accessory subunit; POLZ2; REV7; REV7 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UI95
  • Protein Name
    Mitotic spindle assembly checkpoint protein MAD2B
  • Scientific Description
    Boster Bio Anti-Mad2L2 Monoclonal Antibody catalog # M02357. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Identification of a Novel CD8(+) T cell exhaustion-related gene signature for predicting survival in hepatocellular carcinoma.
    Read more

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Figure 1. Western blot analysis of MAD2L2 using anti-MAD2L2 antibody (A02357-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human SH-SY5Y whole cell lysats. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAD2L2 antigen affinity purified polyclonal antibody (Catalog # A02357-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAD2L2 at approximately 24 kDa. The expected band size for MAD2L2 is at 24 kDa.
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