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Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.

Anti-MAGED4B Antibody

HPA003554
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMAGED4B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MAGED4B Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID81557
  • Target name
    MAGED4B
  • Target description
    MAGE family member D4B
  • Target synonyms
    melanoma-associated antigen D4, MAGE-D4 antigen, MAGE-E1 antigen, melanoma antigen family D, 4B, melanoma antigen family D4B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96JG8
  • Protein Name
    Melanoma-associated antigen D4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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TargetMAGED4B
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Figure 1. Western blot analysis of MAGED4 using anti-MAGED4 antibody (A16674-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human U2OS whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAGED4 antigen affinity purified polyclonal antibody (Catalog # A16674-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAGED4 at approximately 100 kDa. The expected band size for MAGED4 is at 100 kDa.
Product group Antibodies
References
Boster Bio
Anti-MAGED4 Antibody Picoband(r)A16674-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetMAGED4B
  • SizePrice
Atlas Antibodies
PrEST Antigen MAGED4BAPREST86491
Protein IDQ96JG8
  • SizePrice