Anti-Major capsid protein L1 [H16.1A], Mouse Fab fragment, His-Tagged, kappa

Absolute Antibody

Anti-Major capsid protein L1 [H16.1A], Mouse Fab fragment, His-Tagged, kappa

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This antibody is specific for a conformational epitope covering amino acids 266-297 and 339-365.

The fab version of this antibody was used a neutralization assay, in short both a pre- and post-virus attachment assay was performed on 293TT cells with pseudo virus. The structure of this antibody was determined using cryo-EM (Guan et al., 2015; PMID:25996608). This antibody was used for surface plasmon resonance on virus like particles (Towne et al., 2013; PMID:23159495). This antibody was used for an ELISA on HPV-16 L1wt VLPs. This antibody was used for a neutralization assay on HPV-16 pseudo virions, in short the antibody was incubated with HPV-16 pseudo virions, then an attempt was made to infect cervical carcinoma C33A cells with the mixture. This antibody showed some neutralization (Christensen et al., 2001; PMID:11878901).

Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

H16.1A

Mouse

Major capsid protein L1

Virus

-20°C,2°C to 8°C

12352203