Anti-Manganese transport protein C [305-78-7]

Absolute Antibody

Anti-Manganese transport protein C [305-78-7]

7

The antibody showed reduce S. aureus burden in an infant rat passive protection model and induced neutrophil respiratory burst activity against both Staphyloccocus aureus and Staphyloccocus epidermidis (Anderson et al., 2012; PMID: 22474033). The structure of the antibody in complex with MntC was determined. Epitope mapping was carried out using Hydrogen-Deuterium Exchange Mass Spectrometry, the results were further validated using X-ray crystallography, site-directed mutagenesis and functional studies. The binding of the antibody to MntC was tested using ITC and ELISA. ITC experiments showed that binding of the wild type MntC to the antibody was stoichiometric, the apparent Kd was 1.2 nM, and the binding enthalpy was -19.9 kcal/mol. The specificity of the antibody was confirmed by ELISA analysis, with Kd= 1.3 nM. (Gribenko et al., 2016; PMID: 27689696)

ELISA, Other Application

Crystallography; ELISA; ITC; in vivo; in vitro

Research Use Only

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

-20°C,2°C to 8°C

12352203