Anti-ManLAM [My2F12]

Absolute Antibody

Anti-ManLAM [My2F12]

7

This antibody is specific for alpha1-2 linkage of the mannose-capped lipoarabinomannan (LAM) of Mycobacterium tuberculosis and is capable of distinguishing ManLAM from two different M. tuberculosis strains from PILAM of M. smegmatis in sandwich ELISA on b

This antibody is recommended for the detection of M. tuberculosis lipoarabinomannan (LAM) using ELISA. The binding affinity of the human IgG1 version of this antibody for mannose-capped lipoarabinomannan was found to be Kd= 0.438 nM. This antibody was reported to bind mannose-capped LAM of all the slow-growing pathogenic Mycobacterium species. This antibody can also be used for the immunofluorescent staining of heat-killed M. tuberculosis bacilli fixed by methanol-acetone onto slides. This antibody can be used for the detection of tuberculosis (TB) infection via IF sputum smears. This antibody was also used in the detection of mannose-capped LAM in TB patients serum and urine samples (PMID: 25975873).

ImmunoFluorescence, ELISA

ELISA; IF

Research Use Only

Monoclonal

My2F12

lprG

lipoprotein LprG

P27; Rv1411c

Mouse

IgM

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

Bacteria

Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium marinum, Mycobacterium malmoense, Mycobacterium scrofulaceum, Mycobacterium simiae, Mycobacterium xenopi

The original antibody was isolated from a non-immune human Fab-antibody phage display library by multiple rounds of screening against Mycobacterium tuberculosis mannose-capped lipoarabinomannan (LAM) combined with negative depletion on M. smegmatis PILAM.

-20°C,2°C to 8°C

12352203