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Immunohistochemical staining of human cerebellum shows weak cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows weak cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows weak cytoplasmic positivity in Purkinje cells.

Anti-MANSC1 Antibody

HPA007956
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMANSC1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MANSC1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54682
  • Target name
    MANSC1
  • Target description
    MANSC domain containing 1
  • Target synonyms
    9130403P13Rik, LOH12CR3, MANSC domain-containing protein 1, loss of heterozygosity 12 chromosomal region 3 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H8J5
  • Protein Name
    MANSC domain-containing protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MANSC1 using anti-MANSC1 antibody (A15720-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MANSC1 antigen affinity purified polyclonal antibody (Catalog # A15720-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MANSC1 at approximately 62 kDa. The expected band size for MANSC1 is at 62 kDa.
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Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MANSC1 Polyclonal Antibody, Unconjugated (bs-18656R) at 1:400 overnight at 4°C, DAB staining.
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