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Immunohistochemical staining of human fallopian tube shows strong membranous positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows strong membranous positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows strong membranous positivity in glandular cells.

Anti-MANSC4 Antibody

HPA039454
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMANSC4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MANSC4 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID100287284
  • Target name
    MANSC4
  • Target description
    MANSC domain containing 4
  • Target synonyms
    MANSC domain-containing protein 4, MANSC domain-containing protein ENSP00000370673
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDA6NHS7
  • Protein Name
    MANSC domain-containing protein 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MANSC4 using anti-MANSC4 antibody (A19343). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse kidney tissue lysates. Lane 6: mouse HBZY whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MANSC4 antigen affinity purified polyclonal antibody (Catalog # A19343) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MANSC4 at approximately 45 kDa. The expected band size for MANSC4 is at 38 kDa.
Product group Antibodies
Boster Bio
Anti-MANSC4 Antibody Picoband(r)A19343-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetMANSC4
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PrEST Antigen MANSC4APREST81257
Protein IDA6NHS7
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Immunohistochemical staining of human colon shows strong cytoplasmic and luminal membranous positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-MANSC4 AntibodyHPA039682
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetMANSC4
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