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Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Anti-MAP1S Antibody

HPA050934
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetMAP1S
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MAP1S
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55201
  • Target name
    MAP1S
  • Target description
    microtubule associated protein 1S
  • Target synonyms
    BPY2-interacting protein 1; BPY2IP1; C19orf5; MAP-1S; MAP8; microtubule-associated protein 1S; microtubule-associated protein 8; variable charge Y chromosome 2-interacting protein 1; VCY2-interacting protein 1; VCY2IP1; VCY2IP-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ66K74
  • Protein Name
    Microtubule-associated protein 1S
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAP1S using anti-MAP1S antibody (A04308). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP1S antigen affinity purified polyclonal antibody (Catalog # A04308) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP1S at approximately 150 kDa. The expected band size for MAP1S is at 112 kDa.
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