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Immunohistochemical staining of human cerebral cortex shows cytoplasmic immunoreactivity in neurons, as well as positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows cytoplasmic immunoreactivity in neurons, as well as positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows cytoplasmic immunoreactivity in neurons, as well as positivity in neuropil.

Anti-MAP2 Antibody

AMAB91375
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMAP2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MAP2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL5420
  • Conjugate
    Unconjugated
  • Gene ID4133
  • Target name
    MAP2
  • Target description
    microtubule associated protein 2
  • Target synonyms
    MAP-2; MAP2A; MAP2B; MAP2C; microtubule-associated protein 2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP11137
  • Protein Name
    Microtubule-associated protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of MAP2 using anti-MAP2 antibody (A01201-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2 antigen affinity purified polyclonal antibody (Catalog # A01201-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2 at approximately 280 kDa. The expected band size for MAP2 is at 200 kDa.
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