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Anti-MAP4K1/HPK1 Antibody

ORB669235
Biorbyt
ApplicationsWestern Blot
Product group Antibodies
ReactivityRat
TargetMap4k1
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Anti-MAP4K1/HPK1 Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    Western blot, 0.25-0.5microg/ml, Rat. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID292763
  • Target name
    Map4k1
  • Target description
    mitogen activated protein kinase kinase kinase kinase 1
  • Target synonyms
    Hpk1, mitogen-activated protein kinase kinase kinase kinase 1, hematopoietic progenitor kinase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDD3Z8I4
  • Protein Name
    Mitogen-activated protein kinase kinase kinase kinase
  • Scientific Description
    Anti-MAP4K1/HPK1 Antibody. Tested in WB applications. This antibody reacts with Rat.
  • Reactivity
    Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Related products

Figure 1. Western blot analysis of MAP4K1/HPK1 using anti-MAP4K1/HPK1 antibody (A07909-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat NRK whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K1/HPK1 antigen affinity purified polyclonal antibody (Catalog # A07909-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K1/HPK1 at approximately 97KD. The expected band size for MAP4K1/HPK1 is at 97KD.
Product group Antibodies
Boster Bio
Anti-MAP4K1/HPK1 Antibody Picoband(r)A07909-2-CARRIER-FREE
ApplicationsWestern Blot
ReactivityRat
TargetMap4k1
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