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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells, subset of glial cells and in neuropil.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells, subset of glial cells and in neuropil.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells, subset of glial cells and in neuropil.

Anti-MAPK11 Antibody

HPA045069
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetMAPK11
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MAPK11
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5600
  • Target name
    MAPK11
  • Target description
    mitogen-activated protein kinase 11
  • Target synonyms
    MAP kinase 11; MAP kinase p38 beta; mitogen-activated protein kinase 11; mitogen-activated protein kinase p38 beta; mitogen-activated protein kinase p38-2; p38-2; P38B; p38Beta; P38BETA2; PRKM11; SAPK2; SAPK2B; stress-activated protein kinase-2; stress-activated protein kinase-2b
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15759
  • Protein Name
    Mitogen-activated protein kinase 11
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAPK11 using anti-MAPK11 antibody (A03738-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human SH-5Y5Y whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: human K562 whole cell lysates, Lane 8: rat lung tissue lysates, Lane 9: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK11 antigen affinity purified polyclonal antibody (Catalog # A03738-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPK11 at approximately 45KD. The expected band size for MAPK11 is at 45KD.
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