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Anti-MAPK6/ERK3 Antibody

Anti-MAPK6/ERK3 Antibody

A83654
Antibodies.com
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-MAPK6 Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Host
    Goat
  • Isotype
    IgG
  • Scientific Description
    Goat polyclonal antibody to MAPK6/ERK3.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAPK6 using anti-MAPK6 antibody (PB9666). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug, Lane 3: PANC Whole Cell Lysate at 40ug, Lane 4: NIH3T3 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK6 antigen affinity purified polyclonal antibody (Catalog # PB9666) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPK6 at approximately 100 kDa. The expected band size for MAPK6 is at 68 kDa.
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