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Immunohistochemical staining of human lymph node shows moderate to strong nuclear positivity in germinal center cells.
Immunohistochemical staining of human lymph node shows moderate to strong nuclear positivity in germinal center cells.
Immunohistochemical staining of human lymph node shows moderate to strong nuclear positivity in germinal center cells.

Anti-MBD4 Antibody

HPA002031
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMBD4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MBD4 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8930
  • Target name
    MBD4
  • Target description
    methyl-CpG binding domain 4, DNA glycosylase
  • Target synonyms
    MED1, TPDS2, UVM1, methyl-CpG-binding domain protein 4, 3,N(4)-ethenocytosine glycosylase, G/5-fluorouracil mismatch glycosylase with biphasic kinetics, G/T mismatch glycosylase, G/U mismatch glycosylase, methyl-CpG binding domain protein 4, methyl-CpG-binding endonuclease 1, methyl-CpG-binding protein MBD4, mismatch-specific DNA N-glycosylase, putative methyl-CpG binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95243
  • Protein Name
    Methyl-CpG-binding domain protein 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of MBD4/MED1 using anti-MBD4/MED1 antibody (A03462). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SK-OV-3 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human hela whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human MDA-MB-453 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBD4/MED1 antigen affinity purified polyclonal antibody (Catalog # A03462) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBD4/MED1 at approximately 66KD. The expected band size for MBD4/MED1 is at 66KD.
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