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Anti-MBL2 Antibody

ORB2614563
Biorbyt
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMbl2
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Anti-MBL2 Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    Western blot, 0.1-0.5microg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1microg/ml ELISA, 0.1-0.5microg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Biotin
  • Gene ID64668
  • Target name
    Mbl2
  • Target description
    mannose binding lectin 2
  • Target synonyms
    Ab2-001, Ab2-011, mannose-binding protein C, MBP-C, RA-reactive factor P28A subunit, RARF/P28A, mannan-binding protein, mannose binding lectin 2 (protein C), mannose-binding lectin (protein C) 2, mannose-binding protein C (liver), mannose-binding protein C-like, ra-reactive factor polysaccharide-binding component p28A, raRF p28A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08661
  • Protein Name
    Mannose-binding protein C
  • Scientific Description
    Anti-MBL2 Antibody. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MBL2 using anti-MBL2 antibody (A01000-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBL2 antigen affinity purified polyclonal antibody (Catalog # A01000-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBL2 at approximately 26KD. The expected band size for MBL2 is at 26KD.
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