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Anti-MCC (Center) Antibody

102-22660
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetMCC
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-MCC (Center) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB37047
  • Gene ID4163
  • Target name
    MCC
  • Target description
    MCC regulator of Wnt signaling pathway
  • Target synonyms
    MCC1, colorectal mutant cancer protein, MCC, WNT signaling pathway regulator, mutated in colorectal cancers
  • Host
    Rabbit
  • Protein IDP23508
  • Protein Name
    Colorectal mutant cancer protein
  • Scientific Description
    Rabbit Anti-MCC (Center) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MCC using anti-MCC antibody (A00711-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCC antigen affinity purified polyclonal antibody (Catalog # A00711-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCC at approximately 98 kDa. The expected band size for MCC is at 91 kDa.
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