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Immunofluorescent staining of human cell line HeLa shows localization to cytosol.
Immunofluorescent staining of human cell line HeLa shows localization to cytosol.
Immunofluorescent staining of human cell line HeLa shows localization to cytosol.

Anti-MCM3AP Antibody

HPA032103
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetMCM3AP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MCM3AP
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8888
  • Target name
    MCM3AP
  • Target description
    minichromosome maintenance complex component 3 associated protein
  • Target synonyms
    80 kDa MCM3-associated protein; GANP; germinal center-associated nuclear protein; germinal-center associated nuclear protein; germinal-centre associated nuclear protein; MAP80; MCM3 acetylating protein; MCM3 acetyltransferase; MCM3 import protein; MCM3 minichromosome maintenance deficient 3 associated protein; PNRIID; SAC3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60318
  • Protein Name
    Germinal-center associated nuclear protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GANP/MCM3AP using anti-GANP/MCM3AP antibody (A05445-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GANP/MCM3AP antigen affinity purified polyclonal antibody (Catalog # A05445-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GANP/MCM3AP at approximately 218 kDa. The expected band size for GANP/MCM3AP is at 218 kDa.
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