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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Anti-MCM4 Antibody

HPA031052
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetMCM4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MCM4
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4173
  • Target name
    MCM4
  • Target description
    minichromosome maintenance complex component 4
  • Target synonyms
    CDC21, CDC54, IMD54, NKCD, NKGCD, P1-CDC21, hCdc21, DNA replication licensing factor MCM4, homolog of S. pombe cell devision cycle 21, minichromosome maintenance deficient 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP33991
  • Protein Name
    DNA replication licensing factor MCM4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MCM4 using anti-MCM4 antibody (A02301-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human HL-60 whole cell lysates, Lane 7: rat liver tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM4 antigen affinity purified polyclonal antibody (Catalog # A02301-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM4 at approximately 97KD. The expected band size for MCM4 is at 97KD.
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