Figure 1. IHC analysis of MCSF using anti-MCSF antibody (PB9562). MCSF was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-MCSF Antibody (PB9562) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 1. IHC analysis of MCSF using anti-MCSF antibody (PB9562). MCSF was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-MCSF Antibody (PB9562) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Anti-MCSF/CSF1 Antibody
PB9562-CARRIER-FREE
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCsf1
Overview
- SupplierBoster Bio
- Product NameAnti-MCSF/CSF1 Antibody
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID12977
- Target nameCsf1
- Target descriptioncolony stimulating factor 1 (macrophage)
- Target synonymsC87615; Csf; CSF-; Csfm; macrophage colony-stimulating factor 1; M-CS; MCSF; op; osteopetrosis
- HostRabbit
- IsotypeIgG
- Protein IDP07141
- Protein NameMacrophage colony-stimulating factor 1
- Scientific DescriptionBoster Bio Anti-MCSF/CSF1 Antibody Picoband® catalog # PB9562. Tested in ELISA, IHC applications. This antibody reacts with Mouse, Rat.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
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