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Immunohistochemistry analysis in human placenta and pancreas tissues using Anti-MED12 antibody. Corresponding MED12 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human placenta and pancreas tissues using Anti-MED12 antibody. Corresponding MED12 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human placenta and pancreas tissues using Anti-MED12 antibody. Corresponding MED12 RNA-seq data are presented for the same tissues.

Anti-MED12 Antibody

HPA003185
Atlas Antibodies
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMED12
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MED12
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9968
  • Target name
    MED12
  • Target description
    mediator complex subunit 12
  • Target synonyms
    activator-recruited cofactor 240 kDa component; ARC240; CAG repeat protein 45; CAGH45; FGS1; HOPA; human opposite paired; Kohtalo homolog; Kto; MED12S; mediator of RNA polymerase II transcription subunit 12; mediator of RNA polymerase II transcription, subunit 12 homolog; OHDOX; OKS; OPA1; OPA-containing protein; putative mediator subunit 12; thyroid hormone receptor-associated protein complex 230 kDa component; thyroid hormone receptor-associated protein, 230 kDa subunit; TNRC11; TRAP230; trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit); trinucleotide repeat-containing gene 11 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ93074
  • Protein Name
    Mediator of RNA polymerase II transcription subunit 12
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of MED12 using anti-MED12 antibody (A00828-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED12 antigen affinity purified polyclonal antibody (Catalog # A00828-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED12 at approximately 260 kDa. The expected band size for MED12 is at 260 kDa.
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Immunohistochemical staining of human cerebral cortex, colon, spleen and testis using Anti-MED12 antibody HPA003184 (A) shows similar protein distribution across tissues to independent antibody HPA003185 (B).
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