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Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.

Anti-MED14 Antibody

HPA064182
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetMED14
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MED14
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9282
  • Target name
    MED14
  • Target description
    mediator complex subunit 14
  • Target synonyms
    CRSP150, CRSP2, CSRP, CXorf4, DRIP150, EXLM1, RGR1, TRAP170, mediator of RNA polymerase II transcription subunit 14, ARC150, CRSP complex subunit 2, RGR1 homolog, activator-recruited cofactor 150 kDa component, cofactor required for Sp1 transcriptional activation, subunit 2 (150kD), hRGR1, human homolog of yeast RGR1, thyroid hormone receptor-associated protein complex 170 kDa component, thyroid hormone receptor-associated protein complex component TRAP170, transcriptional co-activator CRSP150, vitamin D receptor-interacting protein complex component DRIP150, vitamin D3 receptor-interacting protein complex 150 kDa component
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60244
  • Protein Name
    Mediator of RNA polymerase II transcription subunit 14
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of MED14 using anti-MED14 antibody (A04799-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED14 antigen affinity purified polyclonal antibody (Catalog # A04799-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED14 at approximately 160KD. The expected band size for MED14 is at 160KD.
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