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Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells.

Anti-MED15 Antibody

HPA003179
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMED15
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MED15 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51586
  • Target name
    MED15
  • Target description
    mediator complex subunit 15
  • Target synonyms
    ARC105, CAG7A, CTG7A, PCQAP, TIG-1, TIG1, TNRC7, mediator of RNA polymerase II transcription subunit 15, CTG repeat protein 7a, PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein, PC2 glutamine/Q-rich-associated protein, PC2-glutamine-rich-associated protein, TPA inducible gene-1, TPA inducible protein, TPA-inducible gene 1 protein, activator-recruited cofactor 105 kDa component, activator-recruited cofactor, 105-kD, positive cofactor 2, glutamine/Q-rich-associated protein, trinucleotide repeat containing 7, trinucleotide repeat-containing gene 7 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96RN5
  • Protein Name
    Mediator of RNA polymerase II transcription subunit 15
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of MED15 using anti-MED15 antibody (A03568-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: huamn K562 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED15 antigen affinity purified polyclonal antibody (Catalog # A03568-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED15 at approximately 97 kDa. The expected band size for MED15 is at 87 kDa.
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