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Immunohistochemical staining of human lymph node shows strong nuclear positivity in germinal center cells.
Immunohistochemical staining of human lymph node shows strong nuclear positivity in germinal center cells.
Immunohistochemical staining of human lymph node shows strong nuclear positivity in germinal center cells.

Anti-MEF2B Antibody

HPA004734
Atlas Antibodies
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBORCS8-MEF2B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MEF2B Antibody
  • Delivery Days Customer
    4
  • Applications
    ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4207
  • Target name
    BORCS8-MEF2B
  • Target description
    BORCS8-MEF2B readthrough
  • Target synonyms
    LOC729991-MEF2B, MEF2B, MEF2BNB-MEF2B, RSRFR2, myocyte-specific enhancer factor 2B, LOC729991-MEF2B readthrough, MEF2BNB-MEF2B readthrough, myocyte enhancer factor 2B, serum response factor-like protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02080
  • Protein Name
    Myocyte-specific enhancer factor 2B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of MEF2B using anti-MEF2B antibody (A05743-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse skeletal muscle tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEF2B antigen affinity purified polyclonal antibody (Catalog # A05743-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEF2B at approximately 50 kDa. The expected band size for MEF2B is at 39 kDa.
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