More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-MEK4 (MAP2K4) Mouse Monoclonal Antibody [Clone ID: OTI8A8]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K4.

Anti-MEK4 (MAP2K4) Mouse Monoclonal Antibody [Clone ID: OTI8A8]

M01725-2
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetMAP2K4
100 ul
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-MEK4 (MAP2K4) Mouse Monoclonal Antibody [Clone ID: OTI8A8]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI8A8
  • Concentration
    2.36 mg/ml
  • Gene ID6416
  • Target name
    MAP2K4
  • Target description
    mitogen-activated protein kinase kinase 4
  • Target synonyms
    JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1, dual specificity mitogen-activated protein kinase kinase 4, JNK-activated kinase 1, JNK-activating kinase 1, MAP kinase kinase 4, MAPK/ERK kinase 4, MAPKK 4, MEK 4, SAPK/ERK kinase 1, c-Jun N-terminal kinase kinase 1, c-Jun NH2-terminal kinase kinase 1, stress-activated protein kinase kinase 1
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP45985
  • Protein Name
    Dual specificity mitogen-activated protein kinase kinase 4
  • Scientific Description
    Boster Bio Anti-MAP2K4 (MEK4) mouse monoclonal antibody, clone OTI8A8 (formerly 8A8). Catalog# M01725-2. Tested in FC, IF, IHC, IP, WB. This antibody reacts with Human, Mouse, Rat, Dog.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate All lanes: MAP2K4 antibody at 3.25ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45, 46 kDa Observed band size: 45 kDa
Product group Antibodies
Cusabio
MAP2K4 AntibodyCSB-PA013413LA01HU
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetMAP2K4
  • SizePrice
Product group Antibodies
GeneTex
SEK1 / MKK4 antibody [HL1839]GTX637564
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetMAP2K4
  • SizePrice
Product group Antibodies
MedChem Express
Phospho-MEK4 (Ser80) Antibody (YA176, Rabbit)HY-P80834
TargetMAP2K4
  • SizePrice
Product group Antibodies
Abbexa
JNKK AntibodyABX013493
ApplicationsWestern Blot, ELISA
  • SizePrice
Anti-MAP2K4 Antibody
Product group Antibodies
Antibodies.com
Anti-MAP2K4 AntibodyA42043
ApplicationsWestern Blot
  • SizePrice
Product group Antibodies
RayBiotech
Anti-MAP2K4 Antibody144-07724
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
TargetMAP2K4
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-MKK4 Antibody, Affinity PurifiedA302-657A
ApplicationsImmunoPrecipitation
TargetMAP2K4
  • SizePrice
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm & cell junctions.
Product group Antibodies
Atlas Antibodies
Anti-MAP2K4 AntibodyHPA060074
ApplicationsWestern Blot, ImmunoCytoChemistry
ReactivityHuman
TargetMAP2K4
  • SizePrice
Figure 1. Western blot analysis of MAP2K4 using anti-MAP2K4 antibody (A01725-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K4 antigen affinity purified polyclonal antibody (Catalog # A01725-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K4 at approximately 44KD. The expected band size for MAP2K4 is at 44KD.
Product group Antibodies
Boster Bio
Anti-MEK4/MAP2K4 Antibody Picoband(r)A01725-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetMAP2K4
  • SizePrice
Product group Antibodies
Bioss
Phospho-MEK4 (Ser257) Polyclonal AntibodyBS-3392R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetMAP2K4
  • SizePrice