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ProductsBack/Anti-MEK6/MAP2K6 Antibody Picoband(r)
Anti-MEK6 antibody, PA1921, Western blotting Lane 1: Rat Ovary Tissue Lysate Lane 2: Rat Spleen Tissue Lysate Lane 3: Rat Brain Tissue Lysate Lane 4: Rat Heart Tissue Lysate Lane 5: NIH3I3 Cell Lysate Lane 6: HELA Cell Lysate Lane 7: JRUKAT Cell Lysate Lane 8: MCF-7 Cell Lysate
Anti-MEK6 antibody, PA1921, Western blotting Lane 1: Rat Ovary Tissue Lysate Lane 2: Rat Spleen Tissue Lysate Lane 3: Rat Brain Tissue Lysate Lane 4: Rat Heart Tissue Lysate Lane 5: NIH3I3 Cell Lysate Lane 6: HELA Cell Lysate Lane 7: JRUKAT Cell Lysate Lane 8: MCF-7 Cell Lysate
Anti-MEK6 antibody, PA1921, Western blotting Lane 1: Rat Ovary Tissue Lysate Lane 2: Rat Spleen Tissue Lysate Lane 3: Rat Brain Tissue Lysate Lane 4: Rat Heart Tissue Lysate Lane 5: NIH3I3 Cell Lysate Lane 6: HELA Cell Lysate Lane 7: JRUKAT Cell Lysate Lane 8: MCF-7 Cell Lysate

Anti-MEK6/MAP2K6 Antibody Picoband(r)

PA1921-FITC
Boster Bio
ApplicationsWestern Blot
Product group Antibodies
TargetMAP2K6
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-MEK6/MAP2K6 Antibody Picoband(r)
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: The detection limit for MAP2K6 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    FITC
  • Gene ID5608
  • Target name
    MAP2K6
  • Target description
    mitogen-activated protein kinase kinase 6
  • Target synonyms
    CRCMSL, MAPKK6, MEK6, MKK6, PRKMK6, SAPKK-3, SAPKK3, dual specificity mitogen-activated protein kinase kinase 6, Colorectal Cancer Metastasis-Suppressed LncRNA, MAPK/ERK kinase 6, MAPKK 6, MEK 6, SAPK kinase 3, protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6), stress-activated protein kinase kinase 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP52564
  • Protein Name
    Dual specificity mitogen-activated protein kinase kinase 6
  • Scientific Description
    Boster Bio Anti-MEK6/MAP2K6 Antibody catalog # PA1921. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MKK6/MAP2K6 using anti-MKK6/MAP2K6 antibody (A02011-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MKK6/MAP2K6 antigen affinity purified polyclonal antibody (Catalog # A02011-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MKK6/MAP2K6 at approximately 38 kDa. The expected band size for MKK6/MAP2K6 is at 38 kDa.
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