Anti-Melanin [8C3]

Absolute Antibody

Anti-Melanin [8C3]

7

This antibody binds melanin. Melanin is a natural pigment found in most organisms is produced in a specialized group of cells called the melanocytes.

Paracoccidioides brasiliensis is the agent of the human systemic disease paracoccidioidomycosis. The binding characterization of this antibody towards melanin from various organisms was done using ELISA. This antibody was also used in the immunofluorescence analysis of P. brasiliensis melanized yeast cells (with l-DOPA induction) and wild-type conidia (PMID: 21813659). 213Bismuth and 177Lutetium labelled humanized 8C3 antibody was used in the radioimmunotherapy (RIT) of experimental B16-F10 melanoma (PMID: 31323785). It was demonstrated that a combination of humanized anti-melanin antibody conjugated to 213Bismuth and anti-PD-1 ICB reduced tumor growth and increased survival in the Cloudman S91 murine melanoma DBA/2 mouse model (PMID: 31991626). Male Cloudman S91-bearing DBA/2 mice when treated intraperitoneally with a combination of anti-PD-1 alone or low-dose humanized anti-melanin antibody conjugated to177Lutetium RIT alone resulted in modest tumor reduction, while their combination significantly reduced tumor growth and increased survival, suggesting synergy (PMID: 33218169).

ImmunoFluorescence, ELISA, ImmunoHistoChemistry

ELISA; IF; IHC; radioimmunotherapy; immunotherapy

Research Use Only

Monoclonal

8C3

Rabbit

IgG

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Other Species

Species independent

The original antibody was generated by immunizing female BALB/c mice with melanin particles extracted from Paracoccidioides brasiliensis L-DOPA-induced yeast cells suspended in a 1:1 (vol/vol) emulsion of incomplete Freund adjuvant.

-20°C,2°C to 8°C

12352203