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Anti-MEST Antibody

144-66248
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetMEST
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-MEST Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID4232
  • Target name
    MEST
  • Target description
    mesoderm specific transcript
  • Target synonyms
    mesoderm-specific transcript homolog protein; paternally-expressed gene 1 protein; PEG1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5EB52
  • Protein Name
    Mesoderm-specific transcript homolog protein
  • Scientific Description
    MEST pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MEST using anti-MEST antibody (A07241-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEST antigen affinity purified polyclonal antibody (Catalog # A07241-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEST at approximately 43 kDa. The expected band size for MEST is at 40 kDa.
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