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Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells.

Anti-METAP1D Antibody

HPA030298
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetMETAP1D
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-METAP1D
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID254042
  • Target name
    METAP1D
  • Target description
    methionyl aminopeptidase type 1D, mitochondrial
  • Target synonyms
    CDS of metAP-3 within PCR fragment; MAP 1D; MAP1D; metAP 1D; Metap1l; methionine aminopeptidase 1D, mitochondrial; peptidase M 1D
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6UB28
  • Protein Name
    Methionine aminopeptidase 1D, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of METAP1D using anti-METAP1D antibody (A11898-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: rat liver tissue lysates, Lane 8: rat RH35 whole cell lysates, Lane 9: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METAP1D antigen affinity purified polyclonal antibody (Catalog # A11898-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for METAP1D at approximately 37 kDa. The expected band size for METAP1D is at 37 kDa.
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