Anti-metarhodopsin-I [K42-41L]

Absolute Antibody

Anti-metarhodopsin-I [K42-41L]

9

This antibody recognises secifically C3 loop of metarhodopsin-I (residues 230-252) (and also dark-adapted form of rhodopsin) but it does not bind to the ligh-activated form - metarhodopsin-II. Rhodopsin is the best studied GPCR and is present in rod cells

Antibody imprint: This antibody was used to investigate the structure of rhodopsins meta-states; the researchers employed the antibody imprint technique to analyse conformational changes which this molecule undergoes upon activation by light (Bailey et al., 2003). Another group used K42-41L antibody to demonstrate that it is capable of discriminating between metarhodopsins I and II, and that it stabilises metarhodopsin I while inhibiting metarhodopsin II; these data suggest that this antibody might be useful in the structural studies of opsins (Piscitelli et al., 2006). EM ICC: K42-41L antibody was utilised to stain different structures of Xenopus laevis retinas in EM immunocytochemistry LR white samples in a study investigating opsin localisation (Besharse and Wetzel, 1995).

Electron Microscopy, ELISA, ImmunoCytoChemistry

Research Use Only

Monoclonal

K42-41L

RHO

rhodopsin

CSNBAD1; OPN2; opsin 2, rod pigment; opsin-2; rhodopsin; RP4

Mouse

IgG1

Rhodopsin

Bovine, Human

-20°C,2°C to 8°C

12352203