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Immunohistochemical staining of human rectum shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human rectum shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human rectum shows moderate nuclear positivity in glandular cells.

Anti-METTL11B Antibody

HPA028049
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNTMT2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-METTL11B
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID149281
  • Target name
    NTMT2
  • Target description
    N-terminal Xaa-Pro-Lys N-methyltransferase 2
  • Target synonyms
    alpha N-terminal protein methyltransferase 1B; C1orf184; HOMT1B; methyltransferase like 11B; methyltransferase-like protein 11B; METTL11B; NTM1B; X-Pro-Lys N-terminal protein methyltransferase 1B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5VVY1
  • Protein Name
    Alpha N-terminal protein methyltransferase 1B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NTMT2 using anti-NTMT2 antibody (A15877-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NTMT2 antigen affinity purified polyclonal antibody (Catalog # A15877-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NTMT2 at approximately 32 kDa. The expected band size for NTMT2 is at 32 kDa.
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