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Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Anti-METTL17 Antibody

HPA059802
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMETTL17
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-METTL17 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64745
  • Target name
    METTL17
  • Target description
    methyltransferase like 17
  • Target synonyms
    METT11D1, ribosome assembly protein METTL17, mitochondrial, false p73 target gene protein, methyltransferase 11 domain containing 1, methyltransferase 11 domain-containing protein 1, methyltransferase-like protein 17, mitochondrial, protein RSM22 homolog, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H7H0
  • Protein Name
    Ribosome assembly protein METTL17, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of METTL17 using anti-METTL17 antibody (A14339-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METTL17 antigen affinity purified polyclonal antibody (Catalog # A14339-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for METTL17 at approximately 64 kDa. The expected band size for METTL17 is at 51 kDa.
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