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Immunofluorescent staining of human cell line HEK 293 shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line HEK 293 shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line HEK 293 shows localization to the Golgi apparatus.

Anti-METTL26 Antibody

HPA067414
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetMETTL26
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-METTL26
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84326
  • Target name
    METTL26
  • Target description
    methyltransferase like 26
  • Target synonyms
    C16orf13; JFP2; methyltransferase-like 26; UPF0585 protein C16orf13
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96S19
  • Protein Name
    Methyltransferase-like 26
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of METTL26 using anti-METTL26 antibody (A32469-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METTL26 antigen affinity purified polyclonal antibody (Catalog # A32469-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for METTL26 at approximately 23 kDa. The expected band size for METTL26 is at 23 kDa.
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