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Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.

Anti-MFAP1 Antibody

HPA015583
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetMFAP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MFAP1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4236
  • Target name
    MFAP1
  • Target description
    microfibril associated protein 1
  • Target synonyms
    AMP, microfibrillar-associated protein 1, microfibrillar associated protein 1, spliceosome B complex protein MFAP1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55081
  • Protein Name
    Microfibrillar-associated protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of MFAP1 using anti-MFAP1 antibody (A09850-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MFAP1 antigen affinity purified polyclonal antibody (Catalog # A09850-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MFAP1 at approximately 50 kDa. The expected band size for MFAP1 is at 50 kDa.
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