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Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.

Anti-MFSD12 Antibody

HPA043088
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMFSD12
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MFSD12 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID126321
  • Target name
    MFSD12
  • Target description
    major facilitator superfamily domain containing 12
  • Target synonyms
    C19orf28, PP3501, SLC59B1, major facilitator superfamily domain-containing protein 12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6NUT3
  • Protein Name
    Major facilitator superfamily domain-containing protein 12
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of MFSD12 using anti-MFSD12 antibody (A16855-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MFSD12 antigen affinity purified polyclonal antibody (Catalog # A16855-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MFSD12 at approximately 52 kDa. The expected band size for MFSD12 is at 52 kDa.
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