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Immunohistochemical staining of human endometrium shows moderate nuclear positivity in glandular and stromal cells.
Immunohistochemical staining of human endometrium shows moderate nuclear positivity in glandular and stromal cells.
Immunohistochemical staining of human endometrium shows moderate nuclear positivity in glandular and stromal cells.

Anti-MGA Antibody

HPA042278
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetMGA
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MGA
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23269
  • Target name
    MGA
  • Target description
    MAX dimerization protein MGA
  • Target synonyms
    MAD5; MAX dimerization protein 5; MAX gene-associated protein; MGA, MAX dimerization protein; MXD5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IWI9
  • Protein Name
    MAX gene-associated protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MGA using anti-MGA antibody (PB9873). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: MCF-7 whole cell lysates, Lane 3: SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGA antigen affinity purified polyclonal antibody (Catalog # PB9873) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MGA at approximately 334 kDa. The expected band size for MGA is at 336 kDa.
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