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Immunohistochemical staining of human prostate shows moderate to strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human prostate shows moderate to strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human prostate shows moderate to strong granular cytoplasmic positivity in glandular cells.

Anti-MIGA1 Antibody

HPA017589
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMIGA1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MIGA1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID374986
  • Target name
    MIGA1
  • Target description
    mitoguardin 1
  • Target synonyms
    FAM73A, mitoguardin 1, family with sequence similarity 73, member A, protein FAM73A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NAN2
  • Protein Name
    Mitoguardin 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MIGA1 using anti-MIGA1 antibody (A32472). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIGA1 antigen affinity purified polyclonal antibody (Catalog # A32472) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIGA1 at approximately 71 kDa. The expected band size for MIGA1 is at 71 kDa.
Product group Antibodies
Boster Bio
Anti-MIGA1 Antibody Picoband(r)A32472-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetMIGA1
  • SizePrice
Atlas Antibodies
PrEST Antigen MIGA1APREST72319
Protein IDQ8NAN2
  • SizePrice
Product group Antibodies
GeneTex
FAM73A antibodyGTX122522
ApplicationsWestern Blot
TargetMIGA1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
FAM73A Antibody (aa131-180)LS-C205537
ApplicationsWestern Blot
TargetMIGA1
  • SizePrice