Anti-Migis-alpha [29C11]

Absolute Antibody

Anti-Migis-alpha [29C11]

9

This antibody binds the extracellular peptide called the Migis-alpha consisting of 26-32 amino acids towards the N-terminal of the human membrane bound IgA, when the lipid rafts are disrupted.

Membrane bound IgA is associated with Igalpha/Igbeta as the B cell receptor (BCR) complex on mIgA-expressing B cells. The alpha chain of mIgA (malpha) contains an extracellular domain that is reported to be an antigenic site suitable for isotype-specific targeting of mIgA-expressing B cells by antibodies. The mAb binds strongly to synthetic peptides of migis-alpha and to various recombinant proteins containing migis-alpha as revealed by ELISA. Flow cytometric analysis with B cells suggested that the mAb did not bind strongly to mIgA and required a higher concentration for detection. Immunoprecipitation analysis of these mAbs indicated that mIgA could only be pulled down by 29C11 when mIgA-expressing B cells were solubilized by strong detergents, such as sodium dodecyl sulfate (SDS) or when lipid rafts are disrupted (PMID: 21723611).

ImmunoPrecipitation, ELISA

Research Use Only

Monoclonal

29C11

Rabbit

IgG

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203