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Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.

Anti-MIPEP Antibody

HPA031669
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMIPEP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MIPEP Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4285
  • Target name
    MIPEP
  • Target description
    mitochondrial intermediate peptidase
  • Target synonyms
    COXPD31, HMIP, MIP, mitochondrial intermediate peptidase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99797
  • Protein Name
    Mitochondrial intermediate peptidase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of MIPEP using anti-MIPEP antibody (A05926-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIPEP antigen affinity purified polyclonal antibody (Catalog # A05926-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIPEP at approximately 80 kDa. The expected band size for MIPEP is at 80 kDa.
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