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Immunohistochemical staining of human spleen shows nuclear positivity in cells in red and white pulp.
Immunohistochemical staining of human spleen shows nuclear positivity in cells in red and white pulp.
Immunohistochemical staining of human spleen shows nuclear positivity in cells in red and white pulp.

Anti-MIS12 Antibody

HPA044482
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMIS12
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MIS12 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79003
  • Target name
    MIS12
  • Target description
    MIS12 kinetochore complex component
  • Target synonyms
    2510025F08Rik, KNTC2AP, MTW1, hMis12, protein MIS12 homolog, MIS12 homolog, MIS12, MIND kinetochore complex component, homolog, homolog of yeast Mis12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H081
  • Protein Name
    Protein MIS12 homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of MIS12 using anti-MIS12 antibody (A06832-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human Hacat whole cell lysates, Lane 6: human U251 whole cell lysates, Lane 7: human SH-SY5Y whole cell lysates, Lane 8: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIS12 antigen affinity purified polyclonal antibody (Catalog # A06832-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIS12 at approximately 25 kDa. The expected band size for MIS12 is at 24 kDa.
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